Thermococcus

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A Microbial Biorealm page on the genus Thermococcus

Thermococcus chitonophagus Image by K.O.Stetter & R.Rachel, Univ.Regensburg.

Classification

Higher order taxa:

Archaea; Euryarchaeota; Thermococci; Thermococcales; Thermococcaceae

Species:

Thermococcus acidaminovorans, T. aegaeus, T. aggregans, T. alcaliphilus, T. atlanticus, T. barophilus, T. barossii, T. celer, T. chitonophagus, T. coalescens, T. fumicolans, T. gammatolerans, T. gorgonarius, T. guaymasensis, T. hydrothermalis, T. kodakarensis, T. litoralis, T. marinus, T. mexicalis, T. pacificus, T. peptonophilus, T. profundus, T. radiotolerans, T. sibiricus, T. siculi, T. stetteri, T. waimanguensis, T. waiotapuensis, T. zilligii

NCBI: Taxonomy Genome

Description and Significance

Thermococcus is an obligate heterotroph, strictly anaerobic archaeon which grows on organic substrates, primarily in the presence of elemental sulfur (S°), which is reduced to hydrogen sulfide. The genus contains the highest number of characterized isolates. It is highly motile with a polar tuft of multiple flagella. The archaeon grows between 60-100°C, with an optimum temperature of approximately 85°C, and between 5-9 pH (optimum = 6.5). As more genomes of the species are sequenced, it is expected to encode genes responsible for various cellular functions that provide an advantage for these organisms natural high-temperature habitats.

Genome Structure

T. kodakaraensis has been reported as Pyrococcus sp; however, its complete DNA sequence clearly shows its distiction from its relative.Its circular genome is 2,088,737 bp in length with no exochromosomal element, and contains 2,358 coding sequences (CDSs), of which 2,306 have been identified.Of those CDSs identified, 261 are unique to T. kodakaraensis with no significant homology to any known sequences up to date. Also, its G+C content (52%) is much higher than that of Pyrococus species (40-44%), which is a well-known difference between the two genera.

Cell Structure and Metabolism

The cells of Thermococcus kodakaraensis are irregular cocci of 1-2 µm in diameter, and multiply by constriction. Its cell envelope consists of two layers.

Elemental sulfur (as a terminal electron acceptor) is required for heterotrophic growth on yeast extract, tryptone, peptone, and free amino acids (which produces hydrogen sulfide), but not in the presence of sodium thiosulfate pentahydrate, sodium sulfate, sodium nitrate, sodium nitrite or ferric chloride hexahydrate. However, it is able to utilize maltooligosaccharides, cyclodextrins, starch, or pyruvate in the absence of elemental sulfur (which produces molecular hydrogen).

Predicted pyruvate metabolism in Thermococcus kodakaraensis
Image from "Characterization of an archaeal malic enzyme from the hyperthermophilic archaeon Thermococcus kodakaraensis KOD1." by Fukuda et al, Archaea Vol. 1, 2005, p. 294.]

The figure shows an overview of pyruvate metabolism by T. kodakaraensis. Abbreviations: ACS = acetyl-CoA synthetase; AlaAT = alanine aminotransferase; AspAT = aspartate aminotransferase; Fd = ferre-doxin; GDH = glutamate dehydrogenase; MAE = malic enzyme; PCK = phosphoenolpyruvate carboxykinase; POR = pyruvate:ferredoxin oxidoreductase; PPS = phosphoenol pyruvate synthase; and PYK = pyruvate kinase.

The study by Fukuda et al] raises the possibility of the involvement of malic enzyme in carbon metabolism. Currently, it is understood that malate and fumarate are metabolically isolated in the meta-bolism of T. kodakaraensis because linkages between malate and OAA (malate oxido-reductase) are missing in the pathways deduced from the whole genome analysis.

Ecology

The strain of T. kodakaraensis was isolated from a solfatara (102°C, pH 5.8) on Kodakara Island, Kagoshima, Japan. However, some of the other species, such as T. peptonophilus, are isolated from deep-sea hydrothermal areas. In either case, the genus is considered to play a major role in the ecology and metabolic activity of microbial consortia within hot-water ecosystems.

References

Atomi, H., Fukui, T., Kanai, T., Morikawa, M, and Imanaka, T. "Description of Thermococcus kodakaraensis sp. nov., a well studied hyperthermophilic archaeon previously reoprted as Pyrococcus sp. KOD1." April 16, 2004. Archaea 1, p. 263-267.

Fukuda, W., Ismail, Y. S., Fukui, T., Atomi, H., and Imanaka, T. "Characterization of an archaeal malic enzyme from the hyperthermophilic archaeon Thermococcus kodakaraensis KOD1." January 6, 2005. Archaea 1, p. 293-301.

Fukui, T., Atomi, H., Kanai, T., Matsumi, R., Fujiwara, S., and Imanaka, T. "Complete genome sequence of the hyperthermophilic archaeon Thermococcus kodakaraensis KOD1 and comparison with Pyrococcus genomes." February 14, 2005. Genome Research 15. p. 352-363.

Jolivet, E., L'Haridon, S., Corre, E., Forterre, P., Prieur, D. "Thermococcus gammatolerans sp. nov., a hyperthermophilic archaeon from a deep-sea hydrothermal vent that resists ionizing radiation." 2003. International Journal of Systematic and Evolutionary Microbiology 53, p. 847-851.

Miroshnichenko, M. L., Gongadze, G. M., Rainey, F. A., Kostyukova, A. S., Lysenko, A. M., Chernyh, N. A., and Bonch-Osmolovskaya, E. A. "Thermococcus gorgonarius sp. nov. and Thermococcus pacificus sp. nov.: heterotrophic extremely thermophilic archaea from New Zealand submarine hot vents." 1998. International Journal of Systematic Bacteriology 48, p. 23-29.