Pontibacter salisaro: Difference between revisions
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==Classification== | ==Classification== | ||
Domain: Bacteria | |||
Kingdom: Bscterioldetes/chlorobi | |||
Phylum: Bacteroidetes | |||
Class: Cytophagia | |||
Order: Cytophagales | |||
Family: Cytophagaceae | |||
Genus: Pontibacter | |||
[Others may be used. Use [http://www.ncbi.nlm.nih.gov/Taxonomy/ NCBI] link to find] | [Others may be used. Use [http://www.ncbi.nlm.nih.gov/Taxonomy/ NCBI] link to find] | ||
Line 27: | Line 36: | ||
A spade and a glass mason jar to collet the soil sample. | A spade and a glass mason jar to collet the soil sample. | ||
The soil was collected after digging 1 foot below the surface. | The soil was collected after digging 1 foot below the surface. | ||
The location of the soil sample chosen was in a natural ravine off the end of a gated home property | |||
Rainfall was 0.0" and the pressure was 28.53" | |||
The location was mostly Lewisville silty clay at 1-2 percent slopes. | |||
==Description and Significance== | ==Description and Significance== | ||
Line 43: | Line 55: | ||
smooth | smooth | ||
This soil microbe was placed on a lawn of Pseudomonas Aeruginosa, which is a gram negative bacteria, and it had a | This soil microbe was placed on a lawn of Pseudomonas Aeruginosa, which is a gram negative bacteria, and it had a zone of inhibition which indicates antimicrobial capabilities. | ||
==Genome Structure== | ==Genome Structure== | ||
The Genome structure Pontibacter salisaro strain HMC5104 16S ribosomal RNA gene, partial sequence. It is linear, and not much else is known about this specific bacteria as of yet. | |||
MR 19 forwards strain | |||
Max score: 907 | |||
total score: 907 | |||
Query cover: 85% | |||
Indetify: 98% | |||
accension: KC977017.1 | |||
MR 20 reverse | |||
Max score: 1282 | |||
Total score: 1282 | |||
Query Cover: 99% | |||
Indetify: 99% | |||
Accension: KC9677017.1 | |||
S Ribosomal sequence (partial): | |||
1 ctataatgca gtcgaacgga ccttgagact tcggtttctt ggttagtggc gcacgggtgc | |||
61 gtaacgcgta tgcaacctac cttccactgg gggatagccc ggggaaaccc ggattaatac | |||
121 cgcatgacat cgtttgaggg catccgaaga cgatcaaagc tgaggcggtg gaagatgggc | |||
==Cell Structure, Metabolism and Life Cycle== | ==Cell Structure, Metabolism and Life Cycle== | ||
Currently, Pontibacter Salisaro has not been observed for it's cell structure, metabolism and life cycle. | |||
Here are the results from our lab tests. | |||
It had a zone of inhibition against a lawn of Pseudamonas Aeruginosa | |||
TSI: no fermentation however slight acidic | |||
SIM: Positive for motility | |||
Methyl Red: positive: acidic and is capable for mixed acid fermentation | |||
Nitrate: positive change from Nitrate to Nitrite | |||
Blood agar: alpha hemolysis which refers to partial hemolytic breakdown | |||
PEA test: Gram negative bacteria because inhibition of growth | |||
Bile Esculin and 6.5% salt tolerance test: tested positive for other group D enteroccoci | |||
==Physiology and Pathogenesis== | ==Physiology and Pathogenesis== | ||
Currently, Pontibacter Salisaro has not bee observed in relation to its pathogenic abilities, disinfectants and antibiotic tests were run on agar. | |||
It is resistant to oxacillian. | |||
==Lab notes== | |||
This bacteria was particularly difficult to isolate. I had multiple issues with contamination however upon further review of lab results and research lab book, the contamination could have resulted from not using aseptic techniques long enough in order to kill off any pre-existing bacteria on the loops used, possibly not keeping the covers of the plates and tubes used to culture the microbe closed and/or open for slanted and short periods of time as to not receive air contamination. When looking further into the bacteria there is still not enough available information in journals and books to cross reference information obtained in the lab. It seems this microbe was found in 2011 in South Korea and is still being typed and organized. | |||
==References== | ==References== | ||
1. Joung, Y., Kim, H., Ahn, T.-S., and Joh, K. "Pontibacter salisaro sp. nov., isolated from a clay tablet solar saltern in Korea." J. Microbiol. (2011) 49:290-293. | |||
==Author== | ==Author== |
Latest revision as of 01:53, 8 May 2015
Classification
Domain: Bacteria
Kingdom: Bscterioldetes/chlorobi
Phylum: Bacteroidetes
Class: Cytophagia
Order: Cytophagales
Family: Cytophagaceae
Genus: Pontibacter
[Others may be used. Use NCBI link to find]
Species
NCBI: Taxonomy |
Genus species Pontibacter Salisaro
Habitat Information
Latitude: 30.14402 degrees Longitude: -97.78506 degrees
It was a cloudy day high of 58 degrees low of 44 degrees on Jan 30th 2015. A spade and a glass mason jar to collet the soil sample. The soil was collected after digging 1 foot below the surface. The location of the soil sample chosen was in a natural ravine off the end of a gated home property Rainfall was 0.0" and the pressure was 28.53" The location was mostly Lewisville silty clay at 1-2 percent slopes.
Description and Significance
Describe the appearance (colonial and cellular), possible antimicrobial activity etc. of the organism, and why the organism might be significant.
Cellular: gram negative aerobic rod shaped red pigmented
Colonial: red pigmented round convex smooth
This soil microbe was placed on a lawn of Pseudomonas Aeruginosa, which is a gram negative bacteria, and it had a zone of inhibition which indicates antimicrobial capabilities.
Genome Structure
The Genome structure Pontibacter salisaro strain HMC5104 16S ribosomal RNA gene, partial sequence. It is linear, and not much else is known about this specific bacteria as of yet.
MR 19 forwards strain Max score: 907 total score: 907 Query cover: 85% Indetify: 98% accension: KC977017.1
MR 20 reverse
Max score: 1282
Total score: 1282
Query Cover: 99%
Indetify: 99%
Accension: KC9677017.1
S Ribosomal sequence (partial):
1 ctataatgca gtcgaacgga ccttgagact tcggtttctt ggttagtggc gcacgggtgc 61 gtaacgcgta tgcaacctac cttccactgg gggatagccc ggggaaaccc ggattaatac 121 cgcatgacat cgtttgaggg catccgaaga cgatcaaagc tgaggcggtg gaagatgggc
Cell Structure, Metabolism and Life Cycle
Currently, Pontibacter Salisaro has not been observed for it's cell structure, metabolism and life cycle.
Here are the results from our lab tests.
It had a zone of inhibition against a lawn of Pseudamonas Aeruginosa
TSI: no fermentation however slight acidic
SIM: Positive for motility
Methyl Red: positive: acidic and is capable for mixed acid fermentation
Nitrate: positive change from Nitrate to Nitrite
Blood agar: alpha hemolysis which refers to partial hemolytic breakdown
PEA test: Gram negative bacteria because inhibition of growth
Bile Esculin and 6.5% salt tolerance test: tested positive for other group D enteroccoci
Physiology and Pathogenesis
Currently, Pontibacter Salisaro has not bee observed in relation to its pathogenic abilities, disinfectants and antibiotic tests were run on agar. It is resistant to oxacillian.
Lab notes
This bacteria was particularly difficult to isolate. I had multiple issues with contamination however upon further review of lab results and research lab book, the contamination could have resulted from not using aseptic techniques long enough in order to kill off any pre-existing bacteria on the loops used, possibly not keeping the covers of the plates and tubes used to culture the microbe closed and/or open for slanted and short periods of time as to not receive air contamination. When looking further into the bacteria there is still not enough available information in journals and books to cross reference information obtained in the lab. It seems this microbe was found in 2011 in South Korea and is still being typed and organized.
References
1. Joung, Y., Kim, H., Ahn, T.-S., and Joh, K. "Pontibacter salisaro sp. nov., isolated from a clay tablet solar saltern in Korea." J. Microbiol. (2011) 49:290-293.
Author
Page authored by Wendie Speed_, student of Prof. Kristine Hollingsworth at Austin Community College.