Neorickettsia sennetsu

Classification

Higher order taxa

Kingdom: Bacteria

Phylum: Proteobacteria

Class: Alphaproteobacteria

Order: Rickettsiales

Family: Anaplasmataceae

Genus

Genus: Neorickettsia

Species: sennetsu

NCBI: Taxonomy

Description and significance

When research began on N. sennetsu it was classified as Ehrlichia sennetsu, based on the morphological, ecological, epidemiological and clinical infection characteristics, which were similar to other Erhlichia spp. More recent genetic analysis of the 16S rRna genes, heat shock proteins (groESL), surface proteins, and most recently RNA polymerase beta-subunit have reclassified Ehrlichia sennetsu as Neorickettsia sennetsu. The Anaplasmataceae family now consists of four genera, Ehrlichia, Neorickettsia, Wolbachia and Anaplasma.

Members of the family Anaplasmataceae are Gram-negative obligatory intracellular bacteria, meaning that they cannot survive out side their eukaryotic host cell. The different species within this family are specific to different compartments with in the host cell, such as granulocytes, platelets, endothelial cells, monocytes, macrophages, or red blood cells (9).

N. sennetsu is only infectious to human beings. This disease has only been found in Japan and Malaysia3. Though unknown, the vector of N. sennetsu is thought to be trematodes in fish. The bacteria infect those that eat improperly cooked fish or raw fish (according to the CDC). The bacteria invade monocytes and macrophages. The infection causes Sennetsu fever, which has the following symptoms fever, chills, headache, sore throat, insomnia (4).

The entire genome of all four known strains of N. sennetsu are sequenced.

The following shows (a)the relationship between different species by the sequence of the 16S RNA gene and (b) the infection of a cell by E. sennetsu(5):

(a) (b)

Note: the above pictures were found on the Center for Disease Control website

Genome structure

Total number of DNA molecules: 1; circular chromosome; no plasmids

Total size of all DNA molecules: 859006bp

Total genes: 971

Protein coding genes: 935

tRNA genes: 33

mRNA genes: 3

Cell structure and metabolism

Describe any interesting features and/or cell structures; how it gains energy; what important molecules it produces.

Ecology

Describe any interactions with other organisms (included eukaryotes), contributions to the environment, effect on environment, etc.

Pathology

How does this organism cause disease? Human, animal, plant hosts? Virulence factors, as well as patient symptoms.

Application to Biotechnology

Does this organism produce any useful compounds or enzymes? What are they and how are they used?

Current Research

1. Extensive research on the heat shock proteins HSP60 and HSP70 and their role in E. sennetsu is currently under way. The promoters for the heat shock proteins (HSP) become active in situations when the bacteria live in a higher temperature environment than usual, such as when E. sennetsu infects a human. The similarities between E. sennetsu heat shock proteins and those of other better-known bacteria lead to the belief that the E. sennetsu HSP60 and HSP70 proteins have the same functions. The promoter region for the heat shock 32 sequences lie upstream of the typical 35 and 10 promoter consensus of the 70. This heat shock promoter region is analogous with the 35 promoter region of E. coli heat shock 32 RNA polymerase. In several different and more studied bacteria, including Mycobacterium, Borrelia, Chlamydia, and Legionella spp., the HSP have been recognized by the immune system as common antigens in its response to bacterial infection. Recently, studies have shown bacterial HSP60 and HSP70 to “modulate immunity by directly inducing cytokine mRNA production in macrophages”. HSP70 have also been shown to mediate the role of pathogen and host cell binding. This interaction between host cell and pathogen is critical for N. sennetsu infection because it cannot survive outside host cells. Further research needs to be done to understand the specific role heat shock proteins have in increasing the viability of the bacteria when under thermal stress (2).

References

example:

1. http://cmr.tigr.org/tigr-scripts/CMR/GenomePage.cgi?org=ges

Edited by student of Rachel Larsen and Kit Pogliano