Mycoplasma fermentans: Difference between revisions

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{{Biorealm Genus}}
{{Biorealm Genus}}
==Classification==
==Classification==
Mycoplasma fermentans
[[Image:Mycoplasma_T_celll.png‎|frame|right|100px|[http://www.sciencedirect.com/science/article/pii/S1286457900003956 Figure 1:] Murein macrophage infected by  <I> M. ferementans </I>  shown with an arrow]]
===Higher order taxa===
===Higher order taxa===
Domain (Bacteria), Phylum (Tenericutes), Class (Mollicultes), Order (Myclosplasmales), Family (Mycoplasmatacea), Genus (Mycoplasma), Species (fermentans)
Domain (Bacteria), Phylum (Tenericutes), Class (Mollicultes), Order (Myclosplasmales), Family (Mycoplasmatacea), Genus (Mycoplasma), Species (fermentans)
[http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&id=637387&lvl=3&p=mapview&p=has_linkout&p=blast_url&p=genome_blast&lin=f&keep=1&srchmode=1&unlock NCBI]
[http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&id=637387&lvl=3&p=mapview&p=has_linkout&p=blast_url&p=genome_blast&lin=f&keep=1&srchmode=1&unlock NCBI]
[[Image:Mycoplasma_T_celll.png‎|frame|right|150px|[http://www.sciencedirect.com/science/article/pii/S1286457900003956 Figure 1:] Macrophage infected by  <I> M. ferementans </I>  <sup>1</sup> shown with an arrow]]


===Species===
===Species===
Mycoplasma fermentans
<I> Mycoplasma ferementans </I>


==Description and significance==
==Description and significance==
All mycoplasmas Mycoplasma fermentans are wall-less Gram positive bacteria that colonize human mucosa. M. fermentans are phenotypically distinguished from other bacteria by their small size (0.3-0.8 um in diameter) and lack a cell wall (figure 1). Citation. It is mostly found in the genitourinary and respiratory tracts [2]. It is also known for causing arthritis problem in humans (Vojdani et al., 1998). They are surrounded by a lipoprotein membrane which is the responsible for causing inflammatory reactions [1]. These are pathogens that affect humans and the mechanism of infection is not well known yet. It is also believed that this microbe enhances human immunedeficient virus (HIV) by inducing the viral infection [3].
All mycoplasmas <I> M. ferementans </I> are wall-less Gram positive bacteria that colonize human mucus surfaces <sup>[1]</sup>. <I> M. ferementans </I>are morphologically distinguished from other bacteria by their small size (0.3-0.8 um in diameter) and lack of cell wall <sup>[2]</sup> (figure 1) . It is mostly found in the genitourinary and respiratory tracts <sup>[3]</sup> . It is also known for causing arthritis problem in humans <sup>[3]</sup>. They are surrounded by a lipoprotein membrane which is the responsible for causing inflammatory reactions <sup>[4]</sup>. These are pathogens that affect humans and the mechanism of infection is not well known yet. It is also believed that this microbe enhances human immunedeficient virus (HIV) by inducing the viral infection <sup>[5]</sup>. .[[Image:hand.jpg‎|thumb|500 px|[http://www.medscape.com/content/2004/00/48/77/487710/487710_fig.html Figure 2:] Image of a patient with rheumatoid arthritis  hand]]


==Genome structure==
==Genome structure==
Four strains of M. ferementans M64, JER and PG18, P140 have been isolated, and studied. Based on National Center of Biotechnology Information (NCBI), the entire genome of M. fermentans M64 have been fully sequenced and it is known to have 1,118,751 base pairs that encode for 1050 protein genes, the entire genome is circular (Shu et al., 2011). The genome structure of M. fermentans has been compare with other strain called M. fermentans strain JER (977,524 bp) which is smaller.
Four strains of <I> M. ferementans </I> M64, JER and PG18, P140 have been isolated, characterized, and studied. Based on National Center of Biotechnology Information (NCBI), the entire genome of <I> M. ferementans </I>M64 has been fully sequenced and it is known to have 1,118,751 base pairs that encode for 1050 protein genes, the entire genome is circular <sup>[6]</sup>. The genome structure of <I> M. ferementans </I> has been compare with other strain called M. fermentans strain JER (977,524 bp) which is smaller. It has a low 26.95% [https://www.bio.cmu.edu/courses/03441/TermPapers/99TermPapers/GenEvo/GC-content.htm GC content ], which is important to distiguish mycoplasma among other genus <sup>[7]</sup>.


==Metabolism==
==Metabolism==
Different strains of M. fermentans have been isolated and studied to determine the biochemical variability of this microbe. It is known that this microbe is facultative anaerobe (no oxygen is required for growth but it can grow in the presence of oxygen). There is a lot of variability in the substrate used for metabolic processes. Most of the M. fermentans strains studied until now are able to use glucose during metabolic pathway, but they are also capable of using fructose or arginine, which varies among strains [5].
Different strains of <I> M. ferementans </I> have been isolated and studied to determine the biochemical variability of this microbe <sup>[8]</sup>. It is known that this microbe is facultative anaerobe (no oxygen is required for growth but it can grow in the presence of oxygen). There is a lot of variability in the substrate used for metabolic processes. Most of the <I> M. ferementans </I> strains studied until now are able to use glucose during metabolic pathway, but they are also capable of using fructose or arginine, which varies among strains <sup>[8]</sup>.


==Ecology==
==Ecology==
Mycoplasma fermentans have been isolated from Synovial Fluids of patient with arthritis and also from patients VHI positive, urinary tract and respiratory tract <sup>3, 4, 6</sup>. It have been culture using [https://catalog.hardydiagnostics.com/cp_prod/Content/hugo/SP4Media.htm SP4 media] containing pig serum (inactivated) and a pH of 7.6 <sup>7</sup>. The growth condition is around 37ᵒC, which is the body temperature of its host (humans). [[File:t cell.png|thumb|[https://www.google.com/search?hl=en&biw=1366&bih=667&tbm=isch&q=mycoplasma+fermentans&oq=&gs_l=#imgrc=Xg3zot79Nu1EvM%3A%3BmrrKffDRKOCJ9M%3Bhttp%253A%252F%252Funit.aist.go.jp%252Fbirc2%252Fenglish%252Fimage%252Fmyco1.png%3Bhttp%253A%252F%252Funit.aist.go.jp%252Fbirc2%252Fenglish%252Fother%252Fmyco.html%3B526%3B384 Figure 1:] Macrophage infected by  <I> M. ferementans </I>  <sup>1</sup> shown with an arrow]]
<I> M. ferementans </I> have been isolated from [http://en.wikipedia.org/wiki/Synovial_fluid Synovial Fluids ] of patient with arthritis and also from patients HIV positive, urinary tract and respiratory tract <sup>[3, 4, 6]</sup>. It have been culture using [http://https://catalog.hardydiagnostics.com/cp_prod/Content/hugo/SP4Media.htm SP4 media] containing pig serum (inactivated) and a pH of 7.6 <sup>[8]</sup>. The growth condition is around 37ᵒC, which is the body temperature of its host (humans).   [[File:t cell.png|thumb|[https://www.google.com/search?hl=en&biw=1366&bih=667&tbm=isch&q=mycoplasma+fermentans&oq=&gs_l=#imgrc=Xg3zot79Nu1EvM%3A%3BmrrKffDRKOCJ9M%3Bhttp%253A%252F%252Funit.aist.go.jp%252Fbirc2%252Fenglish%252Fimage%252Fmyco1.png%3Bhttp%253A%252F%252Funit.aist.go.jp%252Fbirc2%252Fenglish%252Fother%252Fmyco.html%3B526%3B384 Figure 3:] T-lymphocyte infected by  <I> M. ferementans </I>  <sup>1</sup> shown with an arrow]]


==Pathology==
==Pathology==
M. fermentans is a pathogenic microbe that affect humans and is usually found in the genital area, and necrotizing tissue (citation). M. fermentans are being linked to diseases like rheumatoid arthritis (citation).  Also studies suggest that M. fermentans causes chronic fatigue in patients (Vojdani et al., 1998). Some of the strains studied like M. fermentans P.140 are known for causing severe respiratory infections and it has been demonstrated that the infection can travel through the blood to other organs such as the kidney, heart, and the brain [6].  It was discovered that during infection M. fermentans attack B cell on the immune system causing inflammatory reactions [1]. In humans it is being investigated that M. fermentans is able to enhance HIV replication, through the lipid-associated proteins present on their membrane [3]. M. fermentans seems to enhance the activation of Long term repeat gene, which is important during HIV infection, thorough a protein called toll receptor [3]
<I> M. ferementans </I> is a pathogenic microbe that affects humans and is usually found in the genital area, and necrotizing tissue <sup>[9]</sup>. <I> M. ferementans </I> are being linked to diseases like [http://en.wikipedia.org/wiki/Rheumatoid_arthritis rheumatoid arthritis]    (figure 2).  Also studies suggest that <I> M. ferementans </I> causes chronic fatigue in patient <sup>[3]</sup>. Some of the strains studied like <I> M. ferementans </I> P.140 are known for causing severe respiratory infections and it has been demonstrated that the infection can travel through the blood to other organs such as the kidney, heart, and the brain <sup>[9]</sup>.  It was discovered that during infection <I> M. ferementans </I> attack cells on the immune system (figure3), such as B cell, causing inflammatory reactions <sup>[4]</sup>. In humans it is being investigated that <I> M. ferementans </I> is able to enhance HIV replication, through the lipid-associated proteins present on their membrane <sup>[5]</sup>. <I> M. ferementans </I> seems to enhance the activation of Long term repeat gene, which is important during HIV infection, thorough a protein called toll receptor <sup>[5]</sup>.


==References==
==References==
1 Rawadi, G. (2000). Mycoplasma fermentans interaction with monocytes/macrophages: molecular basis. Microbes and Infection 2, 955–964.
[1] Marianne in Chinghingyong, Christopher V. Hughes, Detection of Mycoplasma fermentans in human saliva with a polymerase chain reaction-based assay, Archives of Oral Biology, Volume 41, Issue 3, March 1996, Pages 311-314, ISSN 0003-9969, 10.1016/0003-9969(96)84556-0.
2 Vojdani, A., Choppa, P.., Tagle, C., Andrin, R., Samimi, B., and Lapp, C.. (1998). Detection of Mycoplasma genus and Mycoplasma fermentans by PCR in patients with Chronic Fatigue Syndrome. Fems Immunol. Med. Microbiol. 22, 355–365.
(http://www.sciencedirect.com/science/article/pii/0003996996845560)
3 Gerlic, M., Horowitz, J., Farkash, S., and Horowitz, S. (2007). The inhibitory effect of Mycoplasma fermentans on tumour necrosis factor (TNF)-alpha-induced apoptosis resides in the membrane lipoproteins. Cell. Microbiol. 9, 142–153.
 
4 Shimizu, T., Kida, Y., and Kuwano, K. (2004). Lipid-associated membrane proteins of Mycoplasma fermentans and M. penetrans activate human immunodeficiency virus long-terminal repeats through Toll-like receptors. Immunology 113, 121–129.
[2] Stothard P, Van Domselaar G, Shrivastava S, Guo A, O'Neill B, Cruz J, Ellison M, Wishart DS (2005) BacMap: an interactive picture atlas of annotated bacterial genomes. Nucleic Acids Res 33:D317-D320
5 Shu, H.-W., Liu, T.-T., Chan, H.-I., Liu, Y.-M., Wu, K.-M., Shu, H.-Y., Tsai, S.-F., Hsiao, K.-J., Hu, W.S., and Ng, W.V. (2011). Genome Sequence of the Repetitive-Sequence-Rich Mycoplasma fermentans Strain M64▿. J. Bacteriol. 193, 4302–4303.
 
6 Afshar, B., Nicholas, R. a. j., Pitcher, D., Fielder, M. d., and Miles, R. j. (2009). Biochemical and genetic variation in Mycoplasma fermentans strains from cell line, human and animal sources. J. Appl. Microbiol. 107, 498–505.
[3] Vojdani, A., Choppa, P.., Tagle, C., Andrin, R., Samimi, B., and Lapp, C.. (1998). Detection of Mycoplasma genus and Mycoplasma fermentans by PCR in patients with Chronic Fatigue Syndrome. Fems Immunol. Med. Microbiol. 22, 355–365.
7 Yáñez, A., Martínez-Ramos, A., Calixto, T., González-Matus, F.J., Rivera-Tapia, J.A., Giono, S., Gil, C., and Cedillo, L. (2013). Animal model of Mycoplasma fermentans respiratory infection. Bmc Res. Notes 6, 9.
 
[4] Gerlic, M., Horowitz, J., Farkash, S., and Horowitz, S. (2007). The inhibitory effect of Mycoplasma fermentans on tumour necrosis factor (TNF)-alpha-induced apoptosis resides in the membrane lipoproteins. Cell. Microbiol. 9, 142–153.
 
[5] Shimizu, T., Kida, Y., and Kuwano, K. (2004). Lipid-associated membrane proteins of Mycoplasma fermentans and M. penetrans activate human immunodeficiency virus long-terminal repeats through Toll-like receptors. Immunology 113, 121–129.
 
[6] Shu, H.-W., Liu, T.-T., Chan, H.-I., Liu, Y.-M., Wu, K.-M., Shu, H.-Y., Tsai, S.-F., Hsiao, K.-J., Hu, W.S., and Ng, W.V. (2011). Genome Sequence of the Repetitive-Sequence-Rich Mycoplasma fermentans Strain M64▿. J. Bacteriol. 193, 4302–4303.


[7]Rechnitzer, H., Brzuszkiewicz, E., Strittmatter, A., Liesegang, H., Lysnyansky, I., Daniel, R., Gottschalk, G., and Rottem, S. (2011). Genomic features and insights into the biology of Mycoplasma fermentans. Microbiol. Read. Engl. 157, 760–773.


[8] Afshar, B., Nicholas, R. a. j., Pitcher, D., Fielder, M. d., and Miles, R. j. (2009). Biochemical and genetic variation in Mycoplasma fermentans strains from cell line, human and animal sources. J. Appl. Microbiol. 107, 498–505.


[9] Yáñez, A., Martínez-Ramos, A., Calixto, T., González-Matus, F.J., Rivera-Tapia, J.A., Giono, S., Gil, C., and Cedillo, L. (2013). Animal model of Mycoplasma fermentans respiratory infection. Bmc Res. Notes 6, 9.


Edited by Catherine Lobo of Dr. Lisa R. Moore, University of Southern Maine, Department of Biological Sciences, http://www.usm.maine.edu/bio
Edited by Catherine Lobo of Dr. Lisa R. Moore, University of Southern Maine, Department of Biological Sciences, http://www.usm.maine.edu/bio

Latest revision as of 20:55, 3 May 2013

Template in wiki format under LRMoore Prokaryote Template:

This student page has not been curated.

A Microbial Biorealm page on the genus Mycoplasma fermentans

Classification

Figure 1: Murein macrophage infected by M. ferementans shown with an arrow

Higher order taxa

Domain (Bacteria), Phylum (Tenericutes), Class (Mollicultes), Order (Myclosplasmales), Family (Mycoplasmatacea), Genus (Mycoplasma), Species (fermentans) NCBI

Species

Mycoplasma ferementans

Description and significance

All mycoplasmas M. ferementans are wall-less Gram positive bacteria that colonize human mucus surfaces [1]. M. ferementans are morphologically distinguished from other bacteria by their small size (0.3-0.8 um in diameter) and lack of cell wall [2] (figure 1) . It is mostly found in the genitourinary and respiratory tracts [3] . It is also known for causing arthritis problem in humans [3]. They are surrounded by a lipoprotein membrane which is the responsible for causing inflammatory reactions [4]. These are pathogens that affect humans and the mechanism of infection is not well known yet. It is also believed that this microbe enhances human immunedeficient virus (HIV) by inducing the viral infection [5]. .

Figure 2: Image of a patient with rheumatoid arthritis hand

Genome structure

Four strains of M. ferementans M64, JER and PG18, P140 have been isolated, characterized, and studied. Based on National Center of Biotechnology Information (NCBI), the entire genome of M. ferementans M64 has been fully sequenced and it is known to have 1,118,751 base pairs that encode for 1050 protein genes, the entire genome is circular [6]. The genome structure of M. ferementans has been compare with other strain called M. fermentans strain JER (977,524 bp) which is smaller. It has a low 26.95% GC content , which is important to distiguish mycoplasma among other genus [7].

Metabolism

Different strains of M. ferementans have been isolated and studied to determine the biochemical variability of this microbe [8]. It is known that this microbe is facultative anaerobe (no oxygen is required for growth but it can grow in the presence of oxygen). There is a lot of variability in the substrate used for metabolic processes. Most of the M. ferementans strains studied until now are able to use glucose during metabolic pathway, but they are also capable of using fructose or arginine, which varies among strains [8].

Ecology

M. ferementans have been isolated from Synovial Fluids of patient with arthritis and also from patients HIV positive, urinary tract and respiratory tract [3, 4, 6]. It have been culture using SP4 media containing pig serum (inactivated) and a pH of 7.6 [8]. The growth condition is around 37ᵒC, which is the body temperature of its host (humans).

Figure 3: T-lymphocyte infected by M. ferementans 1 shown with an arrow

Pathology

M. ferementans is a pathogenic microbe that affects humans and is usually found in the genital area, and necrotizing tissue [9]. M. ferementans are being linked to diseases like rheumatoid arthritis (figure 2). Also studies suggest that M. ferementans causes chronic fatigue in patient [3]. Some of the strains studied like M. ferementans P.140 are known for causing severe respiratory infections and it has been demonstrated that the infection can travel through the blood to other organs such as the kidney, heart, and the brain [9]. It was discovered that during infection M. ferementans attack cells on the immune system (figure3), such as B cell, causing inflammatory reactions [4]. In humans it is being investigated that M. ferementans is able to enhance HIV replication, through the lipid-associated proteins present on their membrane [5]. M. ferementans seems to enhance the activation of Long term repeat gene, which is important during HIV infection, thorough a protein called toll receptor [5].

References

[1] Marianne in Chinghingyong, Christopher V. Hughes, Detection of Mycoplasma fermentans in human saliva with a polymerase chain reaction-based assay, Archives of Oral Biology, Volume 41, Issue 3, March 1996, Pages 311-314, ISSN 0003-9969, 10.1016/0003-9969(96)84556-0. (http://www.sciencedirect.com/science/article/pii/0003996996845560)

[2] Stothard P, Van Domselaar G, Shrivastava S, Guo A, O'Neill B, Cruz J, Ellison M, Wishart DS (2005) BacMap: an interactive picture atlas of annotated bacterial genomes. Nucleic Acids Res 33:D317-D320

[3] Vojdani, A., Choppa, P.., Tagle, C., Andrin, R., Samimi, B., and Lapp, C.. (1998). Detection of Mycoplasma genus and Mycoplasma fermentans by PCR in patients with Chronic Fatigue Syndrome. Fems Immunol. Med. Microbiol. 22, 355–365.

[4] Gerlic, M., Horowitz, J., Farkash, S., and Horowitz, S. (2007). The inhibitory effect of Mycoplasma fermentans on tumour necrosis factor (TNF)-alpha-induced apoptosis resides in the membrane lipoproteins. Cell. Microbiol. 9, 142–153.

[5] Shimizu, T., Kida, Y., and Kuwano, K. (2004). Lipid-associated membrane proteins of Mycoplasma fermentans and M. penetrans activate human immunodeficiency virus long-terminal repeats through Toll-like receptors. Immunology 113, 121–129.

[6] Shu, H.-W., Liu, T.-T., Chan, H.-I., Liu, Y.-M., Wu, K.-M., Shu, H.-Y., Tsai, S.-F., Hsiao, K.-J., Hu, W.S., and Ng, W.V. (2011). Genome Sequence of the Repetitive-Sequence-Rich Mycoplasma fermentans Strain M64▿. J. Bacteriol. 193, 4302–4303.

[7]Rechnitzer, H., Brzuszkiewicz, E., Strittmatter, A., Liesegang, H., Lysnyansky, I., Daniel, R., Gottschalk, G., and Rottem, S. (2011). Genomic features and insights into the biology of Mycoplasma fermentans. Microbiol. Read. Engl. 157, 760–773.

[8] Afshar, B., Nicholas, R. a. j., Pitcher, D., Fielder, M. d., and Miles, R. j. (2009). Biochemical and genetic variation in Mycoplasma fermentans strains from cell line, human and animal sources. J. Appl. Microbiol. 107, 498–505.

[9] Yáñez, A., Martínez-Ramos, A., Calixto, T., González-Matus, F.J., Rivera-Tapia, J.A., Giono, S., Gil, C., and Cedillo, L. (2013). Animal model of Mycoplasma fermentans respiratory infection. Bmc Res. Notes 6, 9.


Edited by Catherine Lobo of Dr. Lisa R. Moore, University of Southern Maine, Department of Biological Sciences, http://www.usm.maine.edu/bio