Bacillus Anthracis: Anthrax Lethal Toxin: Difference between revisions

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[[Image:cAb29Fig.jpg|thumb|300px|center|Figure 1. A and B show the neutralization assay performed using cultured J774A macrophages that were incubated for 5 h with fixed amounts of LF and PA83 (A) or purified prepore (B) in the presence of cAb29 (circles) or Ab33 (triangles) in the indicated concentrations. Cell survival was determined by XTT and plotted as the percentage of untreated control cells. Points are mean ± S.D. of triplicate determinants. Ab concentration, antibody concentration.]]
[[Image:cAb29Fig.jpg|thumb|300px|center|Figure 1. A and B show the neutralization assay performed using cultured J774A macrophages that were incubated for 5 h with fixed amounts of LF and PA83 (A) or purified prepore (B) in the presence of cAb29 (circles) or Ab33 (triangles) in the indicated concentrations. Cell survival was determined by XTT and plotted as the percentage of untreated control cells. Points are mean ± S.D. of triplicate determinants. Ab concentration, antibody concentration.]]
[[Image:surfactantFig.png|thumb|300px|center|LT decreases surfactant production in AEC. Early-culture AEC were treated with LT (2�g/ml) for 72 h and harvested. Phorbol 12-myristate (PMA; 50 ng/ml) and dexamethasone (Dex; 1�M) were used as positive and negative controls, respectively. Surfactant levels in supernatants were measured by a phospholipid assay and normalized to protein content per sample. Data are means SEM of the results from 3 experiments on 3 separate individuals, with 2 to 4 trials for each treatment. *, P value of �0.05 versus untreated cells.]]




Revision as of 12:23, 23 April 2013

Introduction


By Connor Gibbons

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Legend/credit: Electron micrograph of the Ebola Zaire virus. This was the first photo ever taken of the virus, on 10/13/1976. By Dr. F.A. Murphy, now at U.C. Davis, then at the CDC.
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A photomicrograph of Bacillus anthracis bacteria using Gram-stain technique. Photo obtained from the CDC's Public Health Image Library.
Figure 1. A and B show the neutralization assay performed using cultured J774A macrophages that were incubated for 5 h with fixed amounts of LF and PA83 (A) or purified prepore (B) in the presence of cAb29 (circles) or Ab33 (triangles) in the indicated concentrations. Cell survival was determined by XTT and plotted as the percentage of untreated control cells. Points are mean ± S.D. of triplicate determinants. Ab concentration, antibody concentration.
LT decreases surfactant production in AEC. Early-culture AEC were treated with LT (2�g/ml) for 72 h and harvested. Phorbol 12-myristate (PMA; 50 ng/ml) and dexamethasone (Dex; 1�M) were used as positive and negative controls, respectively. Surfactant levels in supernatants were measured by a phospholipid assay and normalized to protein content per sample. Data are means SEM of the results from 3 experiments on 3 separate individuals, with 2 to 4 trials for each treatment. *, P value of �0.05 versus untreated cells.



Introduce the topic of your paper. What microorganisms are of interest? Habitat? Applications for medicine and/or environment?

Section 1


Include some current research, with at least one figure showing data.

Section 2


Include some current research, with at least one figure showing data.

Section 3


Include some current research, with at least one figure showing data.

Conclusion


Overall text length at least 3,000 words, with at least 3 figures.

References

[Sample reference] Takai, K., Sugai, A., Itoh, T., and Horikoshi, K. "Palaeococcus ferrophilus gen. nov., sp. nov., a barophilic, hyperthermophilic archaeon from a deep-sea hydrothermal vent chimney". International Journal of Systematic and Evolutionary Microbiology. 2000. Volume 50. p. 489-500.

Edited by student of Joan Slonczewski for BIOL 238 Microbiology, 2011, Kenyon College.

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