Snodgrassella alvi wkB2: Difference between revisions
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===Life Cycle=== | ===Life Cycle=== | ||
<i> S. alvi </i> wkB2 lives its entire natural life within the gut of <i> A. mellifera </i>. Cultivation of <i> S. alvi </i> wkB2 in vivo (in <i> A. mellifera </i>) needs no substrate or assistance outside of the bee's regular diet. | |||
This strain can also be cultivated in vitro on the following agars: Heart Infusion, Heart Infusion supplemented with Sheep's Blood, Columbia supplemented with Sheep's Blood (CBA), & R2 Agar. Liquid cultivation of <i> S. alvi </i> wkB2 is typically on Insectargro, Salt-free LB, or R2 Liquid Media. | |||
On solid or liquid media, in vitro cultivation of <i> S. alvi </i> wkB2 is typically at 35°C and 5% CO2. | |||
==Ecology== | ==Ecology== | ||
Revision as of 09:00, 29 April 2020
Classification
Taxonomy
Domain: Bacteria
Phylum: Proteobacteria
Class: Betaproteobacteria
Order: Neisseriales
Family: Neisseriaceae
Genus: Snodgrassella
Species: alvi
Strain: wkB2
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NCBI: Taxonomy |
Names
Snodgrassella alvi wkB2
Candidatus Snodgrassella alvi wkB2 (previously)
S. alvi wkB2
Description and Significance
S. alvi is a core member of the western honey bee ( Apis mellifera ) gut microbiota.
Genome Structure
Genome
The genome of S. alvi wkB2 consists of a singular circular chromosome containing 2,527,978 bp (Kwong 2014). GENES?? Of these genes, 2,299 of them code for proteins. The G+C content is 41.3% (Kwong 2014).
Annotated Genes
A few notable annotated gene functions include genes that code for many pathogen associated functions. One gene codes for nonribosomal peptide synthesis of siderophores, which are small molecules used to sequester iron. The type 6 secretion system (T6SS) which is used to deliver proteins into another cell.
Cell Structure, Metabolism and Life Cycle
Interesting features of cell structure; how it gains energy; what important molecules it produces.
Cell Structure
S. alvi wkB2 is a gram negative microbe. It had a type IV pilli structure and YadA-like adhesins which is used to interact with the host gut epithelium.
Metabolism
S. alvi wkB2 is an obligate aerobe. S. alvi wkB2 has transportation systems for taking up carboxylates like citrate, malate, & α-ketoclutarate. These can then be used in the TAC cycle. Transportation systems within S. alvi wkB2 can also allow for the uptake of lactate which can be directly converted into pyruvate through lactate dehydrogenase.
Lost Metabolic Pathways
Evidence has been found suggesting that S. alvi was able to take up and break down carbohydrates, but has since lost that ability (Kwong 2014). The Glycolosis pathway, Pentose phosphate pathway, & Entner-Doudoroff pathway have all lost essential enzymes (Kwong 2014). These pathways are typically used to convert sugars to pyruvate.
Life Cycle
S. alvi wkB2 lives its entire natural life within the gut of A. mellifera . Cultivation of S. alvi wkB2 in vivo (in A. mellifera ) needs no substrate or assistance outside of the bee's regular diet.
This strain can also be cultivated in vitro on the following agars: Heart Infusion, Heart Infusion supplemented with Sheep's Blood, Columbia supplemented with Sheep's Blood (CBA), & R2 Agar. Liquid cultivation of S. alvi wkB2 is typically on Insectargro, Salt-free LB, or R2 Liquid Media.
On solid or liquid media, in vitro cultivation of S. alvi wkB2 is typically at 35°C and 5% CO2.
Ecology
Habitat; symbiosis; biogeochemical significance; contributions to environment.
For A. mellifera two pests that have been attributed to the large decline in honey bee populations are, the Varroa mite ( Varroa destructor ) and the Deformed Wing Virus (DWV)
A transformed S. alvi wkB2 cells using RNAi have been proposed as a solution to the decreasing honey bee population (Leonard 2020). It has been shown that these synthetic S. alvi wkB2 cells can sucessfully colonize A. mellifera in the same part of the ileum that it usually occupies.
This engineered microbe was transformed with a plasmid coding for double stranded RNA (dsRNA) targeting an essential mRNA on the deformed wing virus which plagues the western honey bee. Leonard et al. were able to show an increase in bee survival for bees incoulated with the DWV and had sucessful colonizations of transformed S. alvi wkB2 cells.
In another experiment, S. alvi wkB2 was transformed with a plasmid which coded for dsRNA which targeted 14 essential Varroa mite genes. They were also able to show a decrease in survival rates for the Varroa mites feeding on these bees.
References
Kwong, W., Engel, P., Koch, H. and Moran, N., 2014. Genomics and host specialization of honey bee and bumble bee gut symbionts. Proceedings of the National Academy of Sciences, 111(31), pp.11509-11514.
Author
Page authored by Samantha Worthington, student of Prof. Jay Lennon at Indiana University.
