CTXφ Bacteriophage: Difference between revisions
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==Overview== | ==Overview== | ||
[[Image:Filamentous_phage.jpg|thumb|230px|right|<b>Figure 1:</b> Filamentous phage(s) under an electron microscope (left), an artist's rendition, and some computer-generated models of varying proteins found in the head, neck, and tail fibers.<ref name = Gagic>Gagic, D., Ciric M., Wen W., Ng F., Rakonjac J. (2016). "Exploring the Secretomes of Microbes and Microbial Communities Using Filamentous Phage Display." Frontiers in Microbiology, 7:429. https://doi.org/10.3389/fmicb.2016.00429.</ref> From <i>Gagic D. et al. (2016)</i>.]] | [[Image:Filamentous_phage.jpg|thumb|230px|right|<b>Figure 1:</b> Filamentous phage(s) under an electron microscope (left), an artist's rendition, and some computer-generated models of varying proteins found in the head, neck, and tail fibers.<ref name = Gagic>Gagic, D., Ciric M., Wen W., Ng F., Rakonjac J. (2016). "Exploring the Secretomes of Microbes and Microbial Communities Using Filamentous Phage Display." Frontiers in Microbiology, 7:429. https://doi.org/10.3389/fmicb.2016.00429.</ref> From <i>Gagic D. et al. (2016)</i>.]] | ||
The CTXφ bacteriophage (or sometimes written as CTX<i>phi</i> bacteriophage) is a lysogenic, filamentous, single-stranded DNA (ssDNA) phage that is responsible for turning the previously non-infectious <i>Vibrio cholerae</i> into a highly pathogenic microbe that causes disease in humans.<ref name = Davis>Davis, B. M., Kimsey, H. H., Chang, W., & Waldor, M. K. (1999). "The Vibrio cholerae O139 Calcutta bacteriophage CTXφ is infectious and encodes a novel repressor." <i>Journal of Bacteriology</i>, 181(21), 6779-6787. https://www.frontiersin.org/articles/10.3389/fmicb.2016.00429/full</ref>,<ref name = Ochman>Ochman, H., Lawrence, J. & Groisman, E. (2000). "Lateral gene transfer and the nature of bacterial innovation." <i>Nature</i>, 405, 299–304. https://doi.org/10.1038/35012500.</ref>, <ref name = Boyd>Boyd, E. F. (2010). "Efficiency and specificity of CTXphi chromosomal integration: dif makes all the difference." Proceedings of the National Academy of Sciences of the United States of America, 107(9), 3951–3952. https://doi.org/10.1073/pnas.1000310107</ref> <i>Vibrio virus</i>CTX<i>phi</i> belongs to the family <i>Inoviridae</i> and the genus <i>Affertcholeramvirus</i>. | The CTXφ bacteriophage (or sometimes written as CTX<i>phi</i> bacteriophage) is a lysogenic, filamentous, single-stranded DNA (ssDNA) phage that is responsible for turning the previously non-infectious <i>Vibrio cholerae</i> into a highly pathogenic microbe that causes disease in humans.<ref name = Davis>Davis, B. M., Kimsey, H. H., Chang, W., & Waldor, M. K. (1999). "The Vibrio cholerae O139 Calcutta bacteriophage CTXφ is infectious and encodes a novel repressor." <i>Journal of Bacteriology</i>, 181(21), 6779-6787. https://www.frontiersin.org/articles/10.3389/fmicb.2016.00429/full</ref>,<ref name = Ochman>Ochman, H., Lawrence, J. & Groisman, E. (2000). "Lateral gene transfer and the nature of bacterial innovation." <i>Nature</i>, 405, 299–304. https://doi.org/10.1038/35012500.</ref>, <ref name = Boyd>Boyd, E. F. (2010). "Efficiency and specificity of CTXphi chromosomal integration: dif makes all the difference." Proceedings of the National Academy of Sciences of the United States of America, 107(9), 3951–3952. https://doi.org/10.1073/pnas.1000310107</ref> <i>Vibrio virus</i> CTX<i>phi</i> belongs to the family <i>Inoviridae</i> and the genus <i>Affertcholeramvirus</i>. | ||
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Revision as of 01:08, 12 December 2020
Overview
The CTXφ bacteriophage (or sometimes written as CTXphi bacteriophage) is a lysogenic, filamentous, single-stranded DNA (ssDNA) phage that is responsible for turning the previously non-infectious Vibrio cholerae into a highly pathogenic microbe that causes disease in humans.[2],[3], [4] Vibrio virus CTXphi belongs to the family Inoviridae and the genus Affertcholeramvirus.
Genetic Material
The CTXφ phage can be identified in its host in both its replicative form (when the bacteriophage is in lysogeny, or the lytic cycle), and in its non-replicative form, which is most common. This phage, when in its lysogenic cycle, integrates its own genetic material in such a way as to form the most stable lysogens.[4]
Infection, Replication & Release from Host Cell
In a process known as lysogenic phage conversion, the CTXφ bacteriophage integrates, among others, its ctxAB genes into its host, Vibrio cholerae. The ctxAB genes code for a type of exotoxin, called Cholera toxin or just "CT," that causes V. cholerae to switch from being nonpathogenic to highly virulent. It is also the primary cause of the large amounts of diarrhea—cholera's main symptom.[4]
Other examples:
Bold
Italic
Subscript: H2O
Superscript: Fe3+
CT & non-CT Toxins
The CTXφ phage contains both types of exotoxins.
CT Toxins
Non-CT Toxins
Conclusion
Overall text length should be at least 1,000 words (before counting references), with at least 2 images. Include at least 5 references under Reference section.
References
- ↑ Gagic, D., Ciric M., Wen W., Ng F., Rakonjac J. (2016). "Exploring the Secretomes of Microbes and Microbial Communities Using Filamentous Phage Display." Frontiers in Microbiology, 7:429. https://doi.org/10.3389/fmicb.2016.00429.
- ↑ Davis, B. M., Kimsey, H. H., Chang, W., & Waldor, M. K. (1999). "The Vibrio cholerae O139 Calcutta bacteriophage CTXφ is infectious and encodes a novel repressor." Journal of Bacteriology, 181(21), 6779-6787. https://www.frontiersin.org/articles/10.3389/fmicb.2016.00429/full
- ↑ Ochman, H., Lawrence, J. & Groisman, E. (2000). "Lateral gene transfer and the nature of bacterial innovation." Nature, 405, 299–304. https://doi.org/10.1038/35012500.
- ↑ 4.0 4.1 4.2 4.3 Boyd, E. F. (2010). "Efficiency and specificity of CTXphi chromosomal integration: dif makes all the difference." Proceedings of the National Academy of Sciences of the United States of America, 107(9), 3951–3952. https://doi.org/10.1073/pnas.1000310107
Edited by Tara Cerny, student of Joan Slonczewski for BIOL 116 Information in Living Systems, 2019, Kenyon College.
