Neorickettsia risticii: Difference between revisions

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==References==
==References==
[1]
[1] []


[2]
[2] [http://www.equinewnv.com/imagefolder/chart-horse-fever-life-cycle.gif]


[3][Chaichanasiriwithaya, W., Rikihisa, Y., Yamamoto, S., Reed, S., Crawford, T., Perryman, L., and Palmer, G. 1994. Antigenic, morphologic, and molecular characterization of new Ehrlichia risticii isolates. Journal of Clinical Microbiology. 38: 3026-3033.]
[3][Chaichanasiriwithaya, W., Rikihisa, Y., Yamamoto, S., Reed, S., Crawford, T., Perryman, L., and Palmer, G. 1994. Antigenic, morphologic, and molecular characterization of new Ehrlichia risticii isolates. Journal of Clinical Microbiology. 38: 3026-3033.]

Revision as of 17:59, 21 November 2011

WIKI IN PROGRESS

Ex. Potomac Horse Fever

Characteristics of the pathogen

Neorickettsia risticii genome [1]

Neorickettsia risticii, formerly called Ehrlichia risticii, belong to the Anaplasmataceae family, the Rickettsiales order, and the Neorickettsia genus. N. risticii was discovered in 1979 during an outbreak of Potomac Horse Fever along the Potomac River in Maryland and Virginia. It is a gram-negative, coccus, and obligate bacterium [3]. N. risticii was demonstrated to be the sole causative agent of Potomac Horse Fever by fulfilling Koch's Postulates in 1985.

The genome for Neorickettsia risticii was fully sequenced in 2009. The Neorickettsia risticii strain sequenced was the Illinois strain, which consists of one circular chromosome that has 879,977 base pairs, with a G-C content of 41.3%. This chromosome encodes for 38 different RNA species, including a 16s RNA, and 898 different proteins [4].

Characteristics of the host

What host/s is/are involved? Is there host specificity? Are there secondary reservoirs?

Life Cycle of Potomac Horse Fever [2]

Host-Symbiont Interaction

The interaction between N. risticii and the horse is a pathogenic one. Infection of a horse with N. risticii causes Potomac Horse Fever, also known as Equine Ehrlichial Colitis, an acute and potentially fatal disease.

N. risticii is an obligate parasite, meaning it cannot survive without a host. It uses a trematode, otherwise known as a fluke, to survive and proliferate [5]. Flukes tend to multiply within snails, and then lay larvae. Or N. risticii uses other aquatic vectors, such as the mayfly or caddisfly, to survive and proliferate [6]. It is through ingestion of fluke larvae or one of these aquatic vectors that the horse becomes infected with N. risticii and contracts Potomac Horse Fever.

Once inside the horse,the bacteria colonizes in the epithelial cells of the horses intestines, monocytes in the blood, and macrophages in the lymphoids and other tissues. N. risticii infection causes many adverse symptoms such as anorexia, fever, depression, diarrhea, lethargy, and in some cases laminitis. N. risticii is also known to cause miscarriages in pregnant horses.

Molecular Insights into the Symbiosis

Describe molecular/genetic studies on the symbiosis.

Ecological and Evolutionary Aspects

What is the evolutionary history of the interaction? Do particular environmental factors play a role in regulating the symbiosis?

Recent Discoveries

Describe two findings on the symbiosis published within the last two years.

References

[1] []

[2] [3]

[3][Chaichanasiriwithaya, W., Rikihisa, Y., Yamamoto, S., Reed, S., Crawford, T., Perryman, L., and Palmer, G. 1994. Antigenic, morphologic, and molecular characterization of new Ehrlichia risticii isolates. Journal of Clinical Microbiology. 38: 3026-3033.]

[4][Lin, M., Zhang, C., Gibson, K., and Rikihisa, Y. 2009. Analysis of complete genome sequence of Neorickettsia risticii: causative agent of potomic horse fever. Nucleic Acid Research. 37 (18): 6076-6091.]

[5][Mott, J., Muramatus, Y., Seaton, E., Martin, C., Reed, S., and Rikihisa, Y. 2002. Molecular analysis of Neorickettsia risticii in adult aquatic insects in Pennsylvania, in horses infected by ingestion of insects, and isolated in cell culture. Journal of Clinical Microbiology. 40(2): 690-693.]

[6][Wilson, J., Pusterla, N., Bengfort, J., and Arney, L. 2006. Incrimination of mayflies as a vector of Potomac horse fever in an outbreak in Minnesota. American Association of Equine Practitioners. 52: 324-328.]

Edited by [Sierra Wangensteen], student of Grace Lim-Fong