Flavivirus NS1 protein

Flavivirus

By Alex Gonzales

Flavivirus Structure

Figure 1. Flavivirus Structure. (A) Schematic representation of a flavivirus particle. Left: immature virion; right: mature virion. The unstructured spherical capsid contains the positive-stranded genomic RNA and multiple copies of the capsid protein C. Immature virions are covered by spiky complexes of 60 trimers of prM-E heterodimers. The proteolytic cleavage of prM results in the reorganization of the E proteins and the formation of smooth-surfaced particles covered with 90 E dimers. sE: soluble form of E that lacks the membrane anchor and an adjacent sequence element called ‘stem’. M: Membrane-associated cleavage product of prM. (B) Herringbone-like arrangement of90 E protein dimers at the virion surface as determined by cryo-electron microscopy .

NS1 Structure

Figure 2. NS1 dimer structure. (A) NS1 dimer with one subunit in gray andthe other colored by domain (blue, b roll; yellow, wing with orange connectorsubdomain; red, central b ladder). Disulfides are shown as yellow spheresand N-linked glycosylation sites as black sticks. A 20-residue disordered regions indicated with dotted lines. C, C terminus; N, N terminus.(Akey et al 2014).

NS1 Expression, post translational processing and trafficking

Figure 3.Schematic summary of NS1 trafficking in mammalian cells.