Serena and Beth - Bacillus thurigiensis
Classification
[1]
Domain: Bacteria
Phylum: Firmicutes
Class: Bacilli
Order: Bacillales
Family :Bacillaceae
Species
NCBI: Taxonomy |
Bacillus
Habitat Information
This organism Bacillus thuringiensis was collected and isolated into a sample of 3 tablespoons of the dampen soil. It was gathered at the soil ground from a homeowner's front lawn on a street named Rei Tang Loop in Kyle, Texas, at the location Latitude: 30.0031643 and Longitude: -97.8756188 and Sea level: 221 m.
The collection laid within a sloped old garden patch that was within a stoned surrounding near a cactus plant and near a home dwelling.
The soil sample was collected on January 28th, 2016. The air temperature was 73 fahrenheit with humidity at 24 percent, with no rainfall prior to 24 hours, with solar radiation being at 2 of 10 UV. Soil was collected from the surface to the depth of 1 inch. The location was collected around 3:30 PM in a semi-shaded semi-sunny location with no foot traffic.
When the organism was observed in growth in an LB plate, at 37 celsius as it's incubated temperature for 168 hours, the organism grew rapidly.
Description and Significance
When isolated and grown in an LB agar plate, Bacillus thuringiensis colonies appeared irregular in shape, slightly umbonate, with smooth entire margins. Under the microscope the Bacillus thuringiensis after performing a Gram stain test results in being a Gram-positive organism, and is rod-shaped in nature and is approximately 1 µm in width and 5 µm in length. When a match patch test was done with 11 other soil samples, Bacillus thuringiensis showed clear evidence of clearing around the colony when grown on an LB plate that contained Staphylococcus aureus and / or Escherichia coli, Including of course the Bacillus thuringiensis itself. This is reason as to why this bacteria was hand pick to focus our studies on since in both scenarios, it suggested possible antimicrobial activity, despite it presenting it's own growth within another bacteria.
Genome Structure
Describe the size and content of the genome. How many chromosomes? Circular or linear? Other interesting features? What is known about its sequence? Include S Ribosomal sequence that you obtained from PCR and sequencing here.
[1]According to American Society for Microbiology , Genome Announcements the total number of predicted genes is 6,635, with 5,714 genes located on the chromosome and 921 genes on the plasmids.
Our PCR sequencing results are as follows:
Sequence 1
Forward:
The following information below contains the number of which was trimmed to eliminate all "N" results and as well as sequencing results.
5' bases trimmed: 19
3' bases trimmed:56
GACGGAGCAACGCCGCGTGAGTGATGAAGGCTT TCGNGGTCGTAAAACTCTGTTGTTAGGGAAGAACAAGTGCTAGTTGAATAAGCTGGCACCTTGACGGTACCTAACCAGAA AGCCACGGCTAACTACGTGCCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTATCCGGAATTATTGGGCGTAAAGCGC GCGCAGGTGGTTTCTTAAGTCTGATGTGAAAGCCCACGGCTCAACCGTGGAGGGTCATTGGAAACTGGGAGACTTGAGTG CAGAAGAGGAAAGTGGAATTCCATGTGTAGCGGTGAAATGCGTAGAGATATGGAGGAACACCAGTGGCGAAGGCGACTTT CTGGTCTGTAACTGACACTGAGGCGCGAAAGCGTGGGGAGCAAACAGGATTAGATACCCTGGTAGTCCACGCCGTAAACG ATGAGTGCTAAGTGTTAGAGGGTTTCCGCCCTTTAGTGCTGAAGTTAACGCATTAAGCACTCCGCCTGGGGAGTACGGCC GCAAGGCTGAAACTCAAAGGAATTGACGGGGGCCCGCACAAGCGGTGGAGCATGTGGTTTAATTCGAAGCAACGCGAAGA ACCTTACCAGGTCTTGACATCCTCTGACAACCCTAGAGATAGGGCTTCTCCTTCGGGAGCAGAGTGACAGGTGGTGCATG GTTGTCGTCAGCTCGTGNCGTGAGATGTCATA
Sequence 2
Reverse:
The following information below contains the number of which was trimmed to eliminate all "N" results and as well as sequencing results.
5' bases trimmed: 12
3' bases trimmed: 26
ACCACCTGTCACTCTGCTCCCGAAGGAGAAGCCCTATCTCTAGGGTTGTCAGAGGATGTCAAGACCTGG TAAGGTTCTTCGCGTTGCTTCGAATTAAACCACATGCTCCACCGCTTGTGCGGGCCCCCGTCAATTCCTTTGAGTTTCAG CCTTGCGGCCGTACTCCCCAGGCGGAGTGCTTAATGCGTTAACTTCAGCACTAAAGGGCGGAAACCCTCTAACACTTAGC ACTCATCGTTTACGGCGTGGACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAGTGTCAGTTACA GACCAGAAAGTCGCCTTCGCCACTGGTGTTCCTCCATATCTCTACGCATTTCACCGCTACACATGGAATTCCACTTTCCT CTTCTGCACTCAAGTCTCCCAGTTTCCAATGACCCTCCACGGTTGAGCCGTGGGCTTTCACATCAGACTTAAGAAACCAC CTGCGCGCGCTTTACGCCCAATAATTCCGGATAACGCTTGCCACCTACGTATTACCGCGGCTGCTGGCACGTAGTTAGCC GTGGCTTTCTGGTTAGGTACCGTCAAGGTGCCAGCTTATTCAACTAGCACTTGTTCTTCCCTAACAACAGAGTTTTACGA CCCGAAAGCCTTCATCACTCACGCGGCGTTGCTCCGTCAGACTTTCGTCCATTGCGGAAGATTCCCTACTGCTGCCTCCC
Cell Structure, Metabolism and Life Cycle
Interesting features of cell structure; how it gains energy; what important molecules it produces.
Physiology and Pathogenesis
Biochemical characteristics, enzymes made, other characteristics that may be used to identify the organism; contributions to environment (if any).
If relevant, how does this organism cause disease? Human, animal, plant hosts? Virulence factors, as well as patient symptoms.
References
Author
Page authored by Serena Perez and Elizabeth Koch, student of Prof. Kristine Hollingsworth at Austin Community College.