Pseudoalteromonas haloplanktis

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A Microbial Biorealm page on the genus Pseudoalteromonas haloplanktis

Classification

Higher order taxa

Bacteria; Proteobacteria; Gammaproteobacteria; Alteromonadales; Pseudoalteromonadaceae; Pseudoalteromonas

A phylogeny tree that describes the 16S rRNA sequences of Pseudoalteromonas species. Looks directly at the class "Proteobacteria", which is one of the higher orders of taxa [1]

Species

P. agarivorans, P. aliena, P. antarctica, P. arctica, P. atlantica, P. aurantia, P. byunsanensis, P. carrageenovora, P. chazhmella, P. citrea, P. denitrificans, P. distincta, P. elyakovii, P. espejiana, P. flavipulchra, P. ganghwensis, P. gracilis, P. haloplanktis, P. issachenkonii, P. luteoviolacea, P. maricaloris, P. marina, P. mariniglutinosa, P. nigrifaciens, P. paragorgicola, P. peptidolyticam P. phenolica, P. piscicida, P. porphyrae, P. prydzensis, P. rubra, P. ruthenica, P. sagamiensis, P. spiralis, P. spongiae, P. telluritireducens, P. tetraodonis, P. translucida, P. tunicate, P. ulvae, P. undina, P. viridis, P. whanghaensis, P. sp.

NCBI: Taxonomy Genome: -P. haloplanktis TAC 125 chromosome 1 -P. haloplanktis TAC 125 chromosome II

Description and significance

Pseudoalteromonas haloplanktis is a curved, rod shaped (0.2 to 1.5 by 1.8 to 3 µm), Antarctica psychrophile bacterium [1]. It was initially called Alteromonas tetraodonis, but has been reclassified as Alteromonas haloplanktis then to Pseudoalteromonas haloplanktis from the phylogenetic assays that was done by [1]. In addition, Pseudoalteromonas haloplanktis is a Gram negative bacterium that is non-spore-forming, not luminescent, and is motile by polar flagella [1]. This organism was isolated from the Antarctic coastal sea sample water and is found to grow only in marine environment at ~20ºC [3]. Researchers have sequenced some of its genome such as DNA ligase and cellulase to give an understanding of how its enzymatic proteins allow it to adapt at such low temperature [1].

Characteristics of the P. haloplanktis genome [2]

Genome structure

Pseudoalteromonas haloplanktis is an aerobe that has no fermentative metabolism. They are rod-shaped bacteria that are classfied as sub species: Pseudoalteromonas haloplanktis subsp. haloplanktis and Pseudoalteromonas haloplanktis subsp. tetraodonis. Strain Pseudoalteromonas haloplanktis TAC 125 is completely sequenced and found to have 2 circular chromosomes, Chromosome I and Chromosome II. Chromosome I has 40% G+C content with 2940 proteins while Chromosome II has 39% G+C rich with 546 proteins. Genes which are encoded account for 87% of the gemone. Read "The complete proteome of Pseudoalteromonas haloplanktis (strain TAC 125)" for further information on the genome of this strain.

A representation of the "Pseudoalteromonas haloplanktis" genome [3]

Cell structure and metabolism

The structure of the lipooligosaccharide (LOS) fraction isolated from P. haloplanktis, consists of a linear-shaped inner core and no O-antigen side chain, emphasize the microorganism's ability to survive in cold conditions [3].P. haloplanktis consists of many cold-adapted enzymes and the majority of them have strong catalytic activities at lower temperatures with minimal activation energy needed[4].

P. haloplanktis is chemoautotroph that requires oxygen; it does not have fermentative metabolism [1]. The marine bacterium, Pseudoalteromonas haloplanktis TAC125, also has metabolic adaptability and has is a good example of a psychrophilic microorganism [4]. The psychrophilic bacteria grow and survive in cold conditions that are usually below 5 °C; Because of these cold conditions the microorganisms have evolved through various intricate adaptations strategies that are focused on the cellular and extra-cellular structures of the bacteria [3]. Pseudoalteromonas haloplanktis DNA ligase has similar catalytic activity to other NAD+-dependent DNA ligases for low-temperature adaptation of organism [10]. The microorganism is seen to be controlled by the functions of its enzymes- the higher the reaction by the catalysed protein, increases the metabolic flow and then the cellular activities occur at low temperature conditions; the need of lower temperatures for enzyme activity is the precise effect that offsets the relationship of the cold temperatures on biochemical reaction outcomes [6].

[8] [7]

Pathology

Oysters are marine eukaryotic hosts for the bacterium, Pseudoalteromonas haloplanktis [7]. The pathologies in fish hosts is not very common but the known toxic species of the bacteria is P. tetraodonis (Pseudoalteromonas haloplanktis tetraodonis), which is found in puffer fish (Fugu poecilonotus) [7]. The bacterial strain creates a potent substance called neurotoxin tetraodontoxin, which is responsible for severe intoxication and deaths reported in humans located in areas of Japan and other countries, through ingestion of insufficiently prepared puffer fish [7].

Application to Biotechnology

Pseudoalteromonas haloplanktis is seen as a valuable biotechnical microorganim because of the ability to grow fast and survive at low temperature conditions in the environment. Research of P. haloplanktis has begun to observe the effects of the bacterium as a new alternative expression host [5]. It has been shown that numerous heterologous enzymes can be produced through the soluble and active states of the bacterium and that P. haloplanktis contains a cold-adapted expression system for recombinant protein production [5].