File:Southernblottanalysis.png

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Revision as of 21:58, 8 May 2013 by Bellk (talk | contribs) (Only one mating type gene pair remains in the somatic nucleus. Southern blot analysis was carried out using whole-cell genomic DNA from a mature strain of each mating type (SB4208, SB4211, SB4214, SB4217, SB4220, and SB4223; see Table S1). The DNA was ...)
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Only one mating type gene pair remains in the somatic nucleus. Southern blot analysis was carried out using whole-cell genomic DNA from a mature strain of each mating type (SB4208, SB4211, SB4214, SB4217, SB4220, and SB4223; see Table S1). The DNA was digested with PvuII restriction endonuclease and separated by pulsed-field gel electrophoresis. Black segments, mating type-specific segment of each gene pair; diagonally hatched segments, conserved TM exons; arrows, PvuII sites; thin black bars, probes; size (kb) shown is that of the relevant PvuII fragment in the somatic genome (the corresponding germline PvuII fragments are not visible due to differences in size and copy number).doi:10.1371/journal.pbio.1001518.g004

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current21:58, 8 May 2013Thumbnail for version as of 21:58, 8 May 20131,944 × 1,707 (836 KB)Bellk (talk | contribs)Only one mating type gene pair remains in the somatic nucleus. Southern blot analysis was carried out using whole-cell genomic DNA from a mature strain of each mating type (SB4208, SB4211, SB4214, SB4217, SB4220, and SB4223; see Table S1). The DNA was ...

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