JJDZ Soil

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Classification

• Domain: Bacteria
• Phylum: Proteobacteria
• Class: Gammaproteobacteria
• Order: Enterobacterales
• Family: Enterobacteriaceae

Species

• Escherichia coli
• NCBI Link: Escherichia coli Taxonomy: [1]

Habitat Information

• Soil Collection Date & time: 9/7/2017 @ 10:00 AM

• Conditions Present at time of soil collection:
  • no recent rain
  • Temperature was 71 degrees F

• Soil sample was retrieved at a depth of roughly 3 inches and about 6-8 inches from creek bed.

• Description of Area:
  • Pond and creek area
  • Moderate erosion
  • Wildlife present (actually saw a coyote when collecting sample)
  • Area was damp and wet (close to creek bed)
  • Area gets roughly 1/2 day of sunlight everyday

Description and Significance

• Colonial Appearance:
  • Shape: Circular
  • Margin: Entire
  • Elevation: Raised
  • Size: Punctiform, small
  • Texture: Smooth
  • Appearance: Shiny
  • Pigmentation: Non-pigmented / colorless
  • Optical Property: Translucent

Describe the appearance (colonial and cellular), possible antimicrobial activity etc. of the organism, and why the organism might be significant.

Genome Structure

• E. coli has only one circular chromosome

Describe the size and content of the genome. How many chromosomes? Circular or linear? Other interesting features? What is known about its sequence? Include S Ribosomal sequence that you obtained from PCR and sequencing here.

Cell Structure, Metabolism and Life Cycle

Interesting features of cell structure; how it gains energy; what important molecules it produces.

Physiology and Pathogenesis

Biochemical characteristics, enzymes made, other characteristics that may be used to identify the organism; contributions to environment (if any).
If relevant, how does this organism cause disease? Human, animal, plant hosts? Virulence factors, as well as patient symptoms.

References

[Sample reference] Takai, K., Sugai, A., Itoh, T., and Horikoshi, K. "Palaeococcus ferrophilus gen. nov., sp. nov., a barophilic, hyperthermophilic archaeon from a deep-sea hydrothermal vent chimney". International Journal of Systematic and Evolutionary Microbiology. 2000. Volume 50. p. 489-500.

Author

Page authored by Jennifer Jordan and Denise Zamarrippa, student of Prof. Kristine Hollingsworth at Austin Community College.