JJDZ Soil: Difference between revisions
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tRNAs :: 90 | tRNAs :: 90 | ||
ncRNAs :: 13 | ncRNAs :: 13 | ||
https://www.ncbi.nlm.nih.gov/nucleotide/1285346620?report=genbank&log$=nuclalign&blast_rank=1&RID=2G0UP0E101R | |||
Describe the size and content of the genome. How many chromosomes? Circular or linear? Other interesting features? What is known about its sequence? Include S Ribosomal sequence that you obtained from PCR and sequencing here. | Describe the size and content of the genome. How many chromosomes? Circular or linear? Other interesting features? What is known about its sequence? Include S Ribosomal sequence that you obtained from PCR and sequencing here. | ||
Revision as of 15:03, 6 December 2017
Classification
- Domain: Bacteria
- Phylum: Proteobacteria
- Class: Gammaproteobacteria
- Order: Enterobacterales
- Family: Enterobacteriaceae
Species
- Escherichia coli
- NCBI Link: Escherichia coli Taxonomy: [1]
Habitat Information
- Soil Sample Location: Copperfield Park and Greenbelt - 1425 E Yager Ln, Austin, TX 78753
- Soil Collection Date & time: 9/7/2017 @ 10:00 AM
- Conditions Present at time of soil collection:
- No recent rain within past 48 hours
- Temperature was 71 degrees F
- Soil sample was retrieved at a depth of roughly 3 inches and about 6-8 inches from creek bed.
- Description of Area:
- Pond and creek area
- Moderate erosion
- Wildlife present (actually saw a coyote when collecting sample)
- Area was damp and wet (close to creek bed)
- Area gets roughly 1/2 day of sunlight everyday
Description and Significance
E. Coli is a Gram-negative, facultatively anaerobic, rod-shaped, coliform bacterium. [1]
- Colonial Appearance:
- Shape: Circular
- Margin: Entire
- Elevation: Raised
- Size: Punctiform, small
- Texture: Smooth
- Appearance: Shiny
- Pigmentation: Non-pigmented / colorless
- Optical Property: Translucent
Describe the appearance (colonial and cellular), possible antimicrobial activity etc. of the organism, and why the organism might be significant.
Genome Structure
- E. coli has only one circular chromosome
- Escherichia coli strain S17-13, complete genome
Genes (total) :: 5,359
CDS (total) :: 5,234
Genes (coding) :: 4,987
CDS (coding) :: 4,987
Genes (RNA) :: 125
rRNAs :: 8, 7, 7 (5S, 16S, 23S)
complete rRNAs :: 8, 7, 7 (5S, 16S, 23S)
tRNAs :: 90
ncRNAs :: 13
https://www.ncbi.nlm.nih.gov/nucleotide/1285346620?report=genbank&log$=nuclalign&blast_rank=1&RID=2G0UP0E101R
Describe the size and content of the genome. How many chromosomes? Circular or linear? Other interesting features? What is known about its sequence? Include S Ribosomal sequence that you obtained from PCR and sequencing here.
Cell Structure, Metabolism and Life Cycle
Interesting features of cell structure; how it gains energy; what important molecules it produces.
Physiology and Pathogenesis
Biochemical characteristics, enzymes made, other characteristics that may be used to identify the organism; contributions to environment (if any).
If relevant, how does this organism cause disease? Human, animal, plant hosts? Virulence factors, as well as patient symptoms.
References
Author
Page authored by Jennifer Jordan and Denise Zamarrippa, student of Prof. Kristine Hollingsworth at Austin Community College.
