JJDZ Soil: Difference between revisions

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==References==
==References==
[Sample reference] [http://ijs.sgmjournals.org/cgi/reprint/50/2/489 Takai, K., Sugai, A., Itoh, T., and Horikoshi, K. "''Palaeococcus ferrophilus'' gen. nov., sp. nov., a barophilic, hyperthermophilic archaeon from a deep-sea hydrothermal vent chimney". ''International Journal of Systematic and Evolutionary Microbiology''. 2000. Volume 50. p. 489-500.]
 
[https://www.ncbi.nlm.nih.gov]


==Author==
==Author==

Revision as of 15:09, 6 December 2017

This student page has not been curated.



Classification

  • Domain: Bacteria
  • Phylum: Proteobacteria
  • Class: Gammaproteobacteria
  • Order: Enterobacterales
  • Family: Enterobacteriaceae

Species

  • Escherichia coli
  • NCBI Link: Escherichia coli Taxonomy: [1]

Habitat Information

caption
  • Soil Sample Location: Copperfield Park and Greenbelt - 1425 E Yager Ln, Austin, TX 78753
  • Soil Collection Date & time: 9/7/2017 @ 10:00 AM
  • Conditions Present at time of soil collection:
    • No recent rain within past 48 hours
    • Temperature was 71 degrees F
  • Soil sample was retrieved at a depth of roughly 3 inches and about 6-8 inches from creek bed.
  • Description of Area:
    • Pond and creek area
    • Moderate erosion
    • Wildlife present (actually saw a coyote when collecting sample)
    • Area was damp and wet (close to creek bed)
    • Area gets roughly 1/2 day of sunlight everyday

Description and Significance

E. Coli is a Gram-negative, facultatively anaerobic, rod-shaped, coliform bacterium. [1]

  • Colonial Appearance:
    • Shape: Circular
    • Margin: Entire
    • Elevation: Raised
    • Size: Punctiform, small
    • Texture: Smooth
    • Appearance: Shiny
    • Pigmentation: Non-pigmented / colorless
    • Optical Property: Translucent

Describe the appearance (colonial and cellular), possible antimicrobial activity etc. of the organism, and why the organism might be significant.

Genome Structure

  • E. coli has only one circular chromosome
  • Escherichia coli strain S17-13, complete genome
           Genes (total)          :: 5,359
           CDS (total)            :: 5,234
           Genes (coding)         :: 4,987
           CDS (coding)           :: 4,987
           Genes (RNA)            :: 125
           rRNAs                  :: 8, 7, 7 (5S, 16S, 23S)
           complete rRNAs         :: 8, 7, 7 (5S, 16S, 23S)
           tRNAs                  :: 90
           ncRNAs                 :: 13
           

NCBI Link: E. coli Strain S17-13: [2]

Describe the size and content of the genome. How many chromosomes? Circular or linear? Other interesting features? What is known about its sequence? Include S Ribosomal sequence that you obtained from PCR and sequencing here.

Cell Structure, Metabolism and Life Cycle

Interesting features of cell structure; how it gains energy; what important molecules it produces.

Physiology and Pathogenesis

[[Biochemical characteristics, enzymes made, other characteristics that may be used to identify the organism; contributions to environment (if any). If relevant, how does this organism cause disease? Human, animal, plant hosts? Virulence factors, as well as patient symptoms]]

References

[3]

Author

Page authored by Jennifer Jordan and Denise Zamarrippa, student of Prof. Kristine Hollingsworth at Austin Community College.