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Nur' Amirah Mohd Kassim Bench D 09082016

Classification

Higher order taxa

Kingdom: Bacteria
Domain: Bacteria
Phylum: Bacteroidetes
Class: Bacteroidia
Order: Bacteroidales
Family: Porphyromonadaceae
Genus: Porphyromonas

Species

Porphyromonas gingivalis strain 2561 = ATCC 33277= CCUG 25893 = CCUG 25928 = CIP 103683 = DSM 20709 = JCM 12257 = NCTC 11834.

Black pigmentation of Porphyromonas gingivalis on blood agar ^[3]

Description and significance

Porphyromonas gingivalis is a gram negative, rod shaped bacteria that is pathogenic, with no functional role ^[1]. It is commonly found in the oral cavity^[2]. On blood agar plate, it forms black pigmented colonies ^[3]. P. gingivalis is the main source of periodontal disease and is also said to be associated with cardiovascular disease amongst other inflammatory disease thus it is important to study this bacterium ^[4].

Examples of citations ^[1], ^[2]

Genome structure

In 2003, the genome of p. gingivalis was described to have 2,343,479bp with an average guanine cytosine nucleotide content of 48.3%. It has 4 ribosomal operons, 2 structural genes and 53 tRNA gene. There was a total of 1990 open reading frames that could be identified in the genome. 21 areas of the genome were discovered to display an atypical nucleotide composition. The areas range in size from 11 to 68kb and has a guanine cytosine nucleotide content of between 29.4% to 61.6%. 463 genes were also seen in the genome ^[5]^[6]

Cell structure and metabolism

Cell wall

biofilm formation

motility

metabolic functions

Ecology

Aerobe/anaerobe, habitat (location in the oral cavity, potential other environments) and microbe/host interactions.

‘’P. gingivalis’’ is an anaerobic rod shaped microorganism that is mainly found in the subgingival plaque of the periodontal of the oral cavity. Other potential environment where the pathogenic bacteria can be found include

Pathology

Do these microorganisms cause disease in the oral cavity or elsewhere?

Application to biotechnology

Bioengineering, biotechnologically relevant enzyme/compound production, drug targets,…

Current research

Summarise some of the most recent discoveries regarding this species.

References

References examples

1. Sahm, K., MacGregor, B.J., Jørgensen, B.B., and Stahl, D.A. (1999) Sulphate reduction and vertical distribution of sulphate-reducing bacteria quantified by rRNA slotblot hybridization in a coastal marine sediment. Environ Microbiol 1: 65-74.

2. Human Oral Microbiome

This page is written by Nur' Amirah Mohd Kassim for the MICR3004 course, Semester 2, 2016